Anna-Louise Cole was born in Melbourne. She holds a BA Hons and a BMus Hons from the University of Melbourne majoring in Music Performance (Voice) and German, and also studied in Austria and Germany, through scholarships from the Deutsche Akademische Austauschdienst and Melbourne Abroad.

Anna-Louise made her debut with Melbourne Opera as Zerlina in Don Giovanni in 2006, and has subsequently performed roles including Erste Dame (Die Zauberflöte), Konstanze (Die Entführung aus dem Serail), Frasquita (Carmen), Serpina (La Serva Padrona), Suor Dolcina (Suor Angelica), Annina (La Traviata), Johanna (Sweeney Todd), der Friedensbote (Rienzi) and Cherubino (La Nozze di Figaro).

Anna-Louise has appeared as a chorus member and soloist with companies including Opera Australia, Victorian Opera, Melbourne Opera, Melbourne Lyric Opera, More Than Opera, the AIMS Festival Orchestra and others in both Austria and Australia, as well as the Kronos Quartet as the soprano soloist in the Australian premiere of Sun Rings for the Melbourne Festival in 2011.

Anna-Louise has won prizes and been a finalist in a number of national and international singing competitions, including the International Hans Gabor Belvedere Singing Competition in Vienna, the Lady Fairfax New York Scholarship, the Audi German Opera Scholarship, the Acclaim Awards and the Herald Sun Aria, and won the American Institute of Musical Studies Award and the Sundell Study Award from Opera Foundation Australia in 2008.

Anna-Louise has worked privately and in masterclasses with major Australian and international artists including Richard Bonynge, AC CBE, Yvonne Kenny AM, Barbara Bonney, David Aronson, Philip Mayers, Bodo Igesz, Richard Divall OBE AO, Sylvia Greenberg, Gabriele Lechner, Sharolyn Kimmorley, Anna Connolly, Raymond Connell and Rosamund Illing. She is currently in the studio of Raymond Lawrence. In 2014 she will be appearing in both Rienzi and La Traviata for Melbourne Opera, and in the choruses of both Carmen and Eugene Onegin for Opera Australia.